Simple, direct determination of serum 5'-nucleotidase.

Colorimetric determination of serum 5'-nucleotidase without deproteinization.

We present a sensitive colorimetric determination of 5'-nucleotidase based on the measurement of liberated phosphate by reaction with stannous chloride/molybdenum blue. No protein precipitation is required. The method is simple, sensitive, economical, and easy to use, and involves relatively stable reagents.

Routine determination of 5' nucleotidase activity of human serum using the LKB 8600 reaction rate analyser.

Determination of serum 5'-nucleotidase with a centrifugal analyzer.

We describe a multi-point method for the determination of the serum enzyme activity 5'-nucleotidase by means of an NADH sensor reaction. Studies show that the assay may be carried out in one step, without preincubation, by using a GEMSAEC Fast Analyzer.

Simple determination of erythrocyte pyrimidine 5'-nucleotidase activity in human blood by high-performance liquid chromatography.

We developed a method for the determination of erythrocyte pyrimidine 5'-nucleotidase (P5N) activity in human blood using a high-performance liquid chromatograph (HPLC). In this method, UMP (uridine 5'-monophosphate disodium salt) was used as a substrate and assay solutions were directly prepared from whole blood. Uridine formed by the enzyme reaction of P5N was determined spectrophotometricall...

A kinetic method for serum 5'-nucleotidase using stabilised glutamate dehydrogenase.

A modification of a kinetic determination of 5'-nucleotidase (EC 3.1.3.5) activity is described. Special attention has been paid to the stabilisation of glutamate dehydrogenase (EC 1.4.1.2) by L-leucine, optimal NADH concentration and the influence of endogeneous ammonia. The optimal concentrations of the other constituents of the reagent were checked with the optimal values given in the litera...